Assessment of microarchitecture and crystal structure of hydroxyapatite in osteoporosis

Universa Medicina

Sutiman B Sumitro¹  Agus Hadian Rahim²  Mohammad Hidayat³  Zairin Noor⁴  Ahmad Taufiq⁵ 

[1] Department of Biology, Faculty of Mathematics and Natural Sciences,Brawijaya University, Malang;Department of Orthopedics, Hasan Sadikin Hospital, Faculty of Medicine, Padjadjaran University, Bandung;Department of Orthopedics, Syaiful Anwar Regional General Hospital,Faculty of Medicine,Brawijaya University, Malang;Department of Orthopedics, Ulin Regional General Hospital, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin;X-ray Diffraction Laboratory, Central Laboratory, Faculty of Mathematics and Natural Sciences,Malang State University;

关键词: Microarchitecture;    hydroxyapatite;    osteoporosis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Osteoporosis is characterized by lower bone mineral density (BMD) and microarchitectural degeneration, which tends to increase bone fragility and fracture risk. Bone microstructure depends on interactions between the mineral atoms, which may perform substitution or incorporation into bone crystals, and may dynamically take over the function of calcium or may become a complementary part. The mineral atoms may also become a composite in the hydroxyapatite crystals. The aim of this study was to find an association between the bone microstructure and hydoxyapatite crystal structure in osteoporosis, in comparison to normal bone. The subjects of this study were surgery patients at the Department of Orthopedics of Ulin General Hospital in Banjarmasin and other centers. Inclusion criteria consisted of the presence of fracture of trabecular bone, normal or osteoporotic BMD values, and no past history of chronic disease. Bone was obtained from fracture patients during surgery. The characteristics of the hydroxyapatite crystals were analyzed by X-ray diffraction (XRD) and the microarchitecture by scanning electron microscopy (SEM). SEM showed degeneration of the microarchitecture of osteoporotic bone, in comparison with normal bone. On XRD there was a peak of hydoxyapatite crystals only and no other crystal phases. Diffraction patterns showed a larger crystal size in osteoporotic bone as compared to normal bone, indicating higher porosity. It may be concluded that there is a difference in crystal size and morphologic distribution of hydoxyapatite in osteoporotic bone, determining bone microarchitecture.

【 授权许可】

Unknown