Aminopropyltriethoxysilane (APTES)-Modified Nanohydroxyapatite (nHAp) Incorporated with Iron Oxide (IO) Nanoparticles Promotes Early Osteogenesis, Reduces Inflammation and Inhibits Osteoclast Activity

Materials

Andrzej Kotela¹  Alina Bienko²  Adrian Patej³  Paulina Sobierajska³  Rafal J. Wiglusz³  Martyna Kepska⁴  Krzysztof Marycz⁴  Eliza Turlej⁴  Katarzyna Kornicka-Garbowska⁴ 

[1] Collegium Medicum, Cardinal Stefan Wyszynski University (UKSW), Woycickiego 1/3, 01-938 Warsaw, Poland;Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie14 Street, 50-383 Wroclaw, Poland;Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland;The Department of Experimental Biology, Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 27B, 50-375 Wroclaw, Poland;

关键词: osteoporosis;    osteoblasts;    osteoclasts;    hydroxyapatite;    APTES;    iron oxides;   

DOI  :  10.3390/ma15062095

来源: DOAJ

【 摘 要 】

Due to its increased prevalence, osteoporosis (OP) represents a great challenge to health care systems and brings an economic burden. To overcome these issues, treatment plans that suit the need of patients should be developed. One of the approaches focuses on the fabrication of personalized biomaterials, which can restore the balance and homeostasis of disease-affected bone. In the presented study, we fabricated nanometer crystalline hydroxyapatite (nHAp) and iron oxide (IO) nanoparticles stabilized with APTES and investigated whether they can modulate bone cell metabolism and be useful in the fabrication of personalized materials for OP patients. Using a wide range of molecular techniques, we have shown that obtained nHAp@APTES promotes viability and RUNX-2 expression in osteoblasts, as well as reducing activity of critical proinflammatory cytokines while inhibiting osteoclast activity. Materials with APTES modified with nHAp incorporated with IO nanoparticles can be applied to support the healing of osteoporotic bone fractures as they enhance metabolic activity of osteoblasts and diminish osteoclasts’ metabolism and inflammation.

【 授权许可】

Unknown