Development of difference spectrophotometric method for the estimation of leflunomide in tablet dosage form

Chemical Industry and Chemical Engineering Quarterly

关键词: leflunomide;    difference spectrophotometric method;    validation;    amplitude;   

DOI  :  10.2298/CICEQ110804016L

来源: DOAJ

【 摘 要 】

A new simple, accurate, precise, highly sensitive and reproducible differencespectrophotometric method for the determination of leflunomide in bulk andpharmaceutical dosage form is described. Difference spectroscopic method isbased on the principle that leflunomide exhibit two different forms; inacidic and basic medium which differs in their absorption spectra. Thedifference spectra were obtained by reading the absorbance of leflunomide in0.1N HCl in the reference cell and the absorbance of leflunomide in 0.1N NaOHin the sample cell and vice versa; in the difference spectrum maxima andminima were seen at 293.5nm and at 261.5nm respectively. The amplitude valueswere calculated, which was plotted against concentration. The method is foundto be linear in the concentration range of 2-12 μg/ml. The percentagerecovery was found to be between the ranges from 98.92 % to 99.08 %. Theproposed method was statistically validated and successfully applied foranalysis of tablet dosage forms.

【 授权许可】

Unknown