Partial structural characterization of pectin cell wall from Argania spinosa leaves

Heliyon

Yasmina Benabdesslem¹  Okkacha Hasnaoui¹  Samia Ghomari²  Kadda Hachem³  Meriem Kaid-Harche³ 

[1] Département de Biologie, Faculté des Sciences, Université Docteur Moulay Tahar de Saida, BP 138 cité ENNASR, 20000 Saida, Algeria;Faculté des Sciences, Université de Djillali Liabès Sidi-Bel-Abbès, BP 89, 22000 Sidi-Bel-Abbés, Algéria;Laboratoire des Productions, Valorisations Végétales et Microbiennes, Département de Biotechnologie, Université des Sciences et de la Technologie d’Oran, BP 1505, El Mnaouar, 31000 Oran, Algeria;

关键词: Biochemistry;    Applied sciences;    Plant biology;    Natural product chemistry;    Biological sciences;   

DOI  :  10.1016/j.heliyon.2016.e00076

来源: DOAJ

【 摘 要 】

The pectin polysaccharides from leaves of Argania spinosa (L.) Skeels, collected from Stidia area in the west coast of northern Algeria, were investigated by using sequential extractions and the resulting fractions were analysed for monosaccharide composition and chemical structure. Water-soluble pectic (ALS-WSP) and chelating-soluble pectic (ALS-CSP) fractions were obtained, de-esterified and fractionated by anion-exchange chromatography and characterized by sugar analysis combined with methylation analysis and 1H and 13C NMR spectroscopy. The data reveal the presence of altering homogalacturonan (HG) and rhamnogalacturonan I (RG-I) in both pectin fraction. The rhamnogalacturonan I (RG-I) are consisted of a disaccharide repeating unit [→ α-D-GalpA-1,2-α-L-Rhap-1,4 →] backbone, with side chains contained highly branched α-(1 → 5)-linked arabinan and short linear β-(1 → 4)-linked galactan, attached to O-4 of the rhamnosyl residues.

【 授权许可】

Unknown