Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

Molecules

Alessandra Braga Ribeiro¹  Luís Manuel Lopes Rodrigues da Silva²  Francisco Lopes da Silva Filho³  Paulo Michel Pinheiro Ferreira⁴  Rayran Walter Ramos de Sousa⁴  Igor Frederico da Silveira Ramos⁵  Ricardo de Araújo⁵  Maurycyo Silva Geronço⁵  Josy Anteveli Osajima⁵  Monalisa de Alencar Lucena⁵  Edson Cavalcanti Silva-Filho⁵  Marcilia Pinheiro da Costa⁵  Márcia dos Santos Rizzo⁶ 

[1] CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal;CPIRN-UDI/IPG—Centro de Potencial e Inovação em Recursos Naturais, Unidade de Investigação para o Desenvolvimento do Interior do Instituto Politécnico da Guarda, 6300-559 Guarda, Portugal;College of Pharmacy, Federal University of Piauí, Teresina 64049-550, PI, Brazil;Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina 64049-550, PI, Brazil;Materials Science and Engineering Graduate Program, Federal University of Piauí, Teresina 64049-550, PI, Brazil;Pharmaceutical Sciences Graduate Program, Federal University of Piauí, Teresina 64049-550, PI, Brazil;

关键词: polysaccharide;    biopolymer;    dextran;    photostability;    mucoadhesiveness;    antimicrobial activity;   

DOI  :  10.3390/molecules27123895

来源: DOAJ

【 摘 要 】

The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 10⁵ Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.

【 授权许可】

Unknown