Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents

photobleaching;photostability;fluorescein;anti-fading agents;hyper-branched polymers

Vincil, Gretchen

关键词: photobleaching;    photostability;    fluorescein;    anti-fading agents;    hyper-branched polymers;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/72771/Gretchen_Vincil.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

The photobleaching of fluorophores is a major limitation in fluorescence microscopy.We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG).The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead.Through the iterative use of ROMP and RCM, organic nanoparticles (ONPs) were synthesized incorporating multiple fluorescein and AFA units.The ONPs were brighter and more photostable than fluorescein.The photostability of the ONPs without AFAs was found to loosely correlate to its brightness.Incorporation of AFAs in the ONPs led to increased stability in some cases.