Leucine Potentiates Glucose-mediated 18F-FDG Uptake in Brown Adipose Tissue via β-Adrenergic Activation

Biomedicines

Brenda Huska¹  SimonJ. Lees¹  Sarah Niccoli²  ChristopherP. Phenix³ 

[1] Biology, Lakehead University, Thunder Bay, ON P7B 5E1, Canada;Medical Sciences, Lakehead University Faculty of Medicine, Thunder Bay, ON P7B 5E1, Canada;Northern Ontario School of Medicine, Medical Sciences Division, Thunder Bay, ON P7B 5E1, Canada;

关键词: positron emission tomography;    non-shivering thermogenesis;    diet-induced thermogenesis;    glucose metabolism;    obesity;    propranolol;   

DOI  :  10.3390/biomedicines8060159

来源: DOAJ

【 摘 要 】

Significant depots of brown adipose tissue (BAT) have been identified in many adult humans through positron emission tomography (PET), with the amount of BAT being inversely correlated with obesity. As dietary activation of BAT has implications for whole body glucose metabolism, leucine was used in the present study to determine its ability to promote BAT activation resulting in increased glucose uptake. In order to assess this, 2-deoxy-2-(fluorine-18)fluoro-d-glucose (¹⁸F-FDG) uptake was measured in C57BL/6 mice using microPET after treatment with leucine, glucose, or both in interscapular BAT (IBAT). Pretreatment with propranolol (PRP) was used to determine the role of β-adrenergic activation in glucose and leucine-mediated ¹⁸F-FDG uptake. Analysis of maximum standardized uptake values (SUV_MAX) determined that glucose administration increased ¹⁸F-FDG uptake in IBAT by 25.3%. While leucine did not promote ¹⁸F-FDG uptake alone, it did potentiate glucose-mediated ¹⁸F-FDG uptake, increasing ¹⁸F-FDG uptake in IBAT by 22.5%, compared to glucose alone. Pretreatment with PRP prevented the increase in IBAT ¹⁸F-FDG uptake following the combination of glucose and leucine administration. These data suggest that leucine is effective in promoting BAT ¹⁸F-FDG uptake through β-adrenergic activation in combination with glucose.

【 授权许可】

Unknown