Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells

International Journal of Molecular Sciences

Matteo Micucci¹  Roberta Budriesi¹  Alberto Chiarini¹  Roberto Gotti¹  Miriam Durante²  Maria Frosini²  Elda Chiaino²  Carla Marzetti³ 

[1] Dipartimento di Farmacia e Biotecnologie, Alma Mater Studiorum, Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy;Dipartimento di Scienze della Vita, Università di Siena, Via Aldo Moro 2, 53100 Siena, Italy;Valsambro S.r.l., Via Cairoli 2, 40121 Bologna, Italy;

关键词: acacia catechu willd.;    colorectal cancer;    apoptosis;    ht-29 cells;    ros;    mitochondrial membrane potential;    catechins;    polyphenols;    natural compounds;   

DOI  :  10.3390/ijms21062102

来源: DOAJ

【 摘 要 】

The research for innovative treatments against colon adenocarcinomas is still a great challenge. Acacia catechu Willd. heartwood extract (AC) has health-promoting qualities, especially at the gastrointestinal level. This study characterized AC for its catechins content and investigated the apoptosis-enhancing effect in human colorectal adenocarcinoma HT-29 cells, along with its ability to spare healthy tissue. MTT assay was used to describe the time course, concentration dependence and reversibility of AC-mediated cytotoxicity. Cell cycle analysis and AV-PI and DAPI-staining were performed to evaluate apoptosis, together with ROS formation, mitochondrial membrane potential (MMP) changes and caspase activities. Rat ileum and colon rings were tested for their viability and functionality to explore AC effects on healthy tissue. Quantitative analysis highlighted that AC was rich in (±)-catechin (31.5 ± 0.82 mg/g) and (−)-epicatechin (12.5 ± 0.42 mg/g). AC irreversibly decreased cell viability in a concentration-dependent, but not time-dependent fashion. Cytotoxicity was accompanied by increases in apoptotic cells and ROS, a reduction in MMP and increases in caspase-9 and 3 activities. AC did not affect rat ileum and colon rings’ viability and functionality, suggesting a safe profile toward healthy tissue. The present findings outline the potential of AC for colon cancer treatment.

【 授权许可】

Unknown