Apoptosis-inducing potential of biosynthesized silver nanoparticles in breast cancer cells

Journal of King Saud University: Science

M. Ajmal Ali¹  Waleed Ali Qaid Hailan²  Khalid Mashai Alanazi³  Ahmed Ali Al-kawmani³  Fahad M.A. Al-Hemaid³  Mohammad Abul Farah³ 

[1] Corresponding author.;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;

关键词: Ochradenus arabicus;    Silver nanoparticles;    Apoptosis;    Autophagy;    Reactive oxygen species;    Cytotoxicity;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanomaterials used in biomedical applications. Moreover, natural products from medicinal plants provide unlimited opportunities for drug development owing to the unmatched availability of a biochemical diversity. In this study, we biosynthesized AgNPs from the leaf extract of Ochradenus arabicus using silver nitrate and evaluated the cytotoxicity and apoptosis inducing ability of the O. arabicus AgNPs (OA-synthesized AgNPs) against the MCF-7 human breast cancer cells. Methods: The OA-synthesized AgNPs were characterized by X-ray diffraction, UV–visible spectroscopy, and transmission electron microscopy. Results: The OA-synthesized AgNPs were highly toxic to the MCF-7 cells as the cell proliferation was significantly decreased post-treatment. The intracellular level of reactive oxygen species (ROS) was significantly increased (p < 0.05). Moreover, a dose-dependent increase in the percentage of apoptosis and autophagy was also evident. Conclusion: The findings of this study confirm the ability of OA-synthesized AgNPs to stimulate apoptosis and suppress cell growth and proliferation.

【 授权许可】

Unknown