Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy

Frontiers in Chemistry

Cong Wu¹  Wanru Tao¹  Xiaohai Yuan¹  Na Wang²  Wei Li³  Weiwei Chu³  Jing Shi³ 

[1] Intentional Joint Cancer Institute, Second Military Medical University, Shanghai, China;Department of Cosmetics, Shanghai Skin Disease Hospital, Shanghai, China;;Laboratory of Nano Biomedicine &

关键词: doxorubicin;    gold nanorods;    thermosensitive;    photothermal therapy;    melanoma;    B16F10;   

DOI  :  10.3389/fchem.2020.599740

来源: DOAJ

【 摘 要 】

Objective: The aim of this study was to construct light and temperature dual-sensitive micellar carriers loaded with doxorubicin (DOX) and gold nanorods (DOX-GNRs-PNIPAM@PEG-PLA, DAPP) for melanoma therapy.Methods: The DAPP self-assembled using fine-tuned physicochemical properties in water. The DAPP structure, temperature- and photo-sensitivity, drug-release, in-vitro serum stability, and cytotoxicity against melanoma B16F10 cells were evaluated in detail. The corresponding in-vitro and in-vivo therapeutic mechanisms were then evaluated using a B16F10-melanoma bearing BALB/c nude mouse model (B16F10).Results: The light and temperature sensitive micellar drug-delivery system assembled from block copolymer and gold nanorods exhibited a narrow particle size and size distribution, good biocompatibility, well-designed photo-temperature conversion, controlled drug release, and high serum stability. Compared with the free DOX- and PBS-treated groups, the cell endocytosis-mediated cytotoxicity and intra-tumor accumulation of DAPP was markedly enhanced by the NIR-light exposure and induced potent in-vivo tumor inhibitory activity.Conclusion: The design of DAPP, a dual-functional micellar drug-delivery system with temperature- and light-sensitive properties, offers a new strategy for skin-cancer therapy with a potent therapeutic index.

【 授权许可】

Unknown