Gold nanorods for target selective SPECT/CT imaging and photothermal therapy in vivo

Quantitative Imaging in Medicine and Surgery

Boseung Jang¹  Yongdoo Choi¹  Seonhwa Park¹  Seok-Ki Kim¹  Se Hun Kang¹  In-Hoo Kim¹  Joa Kyum Kim¹ 

[1] Molecular Imaging & Therapy Branch, Division of Convergence Technology, National Cancer Center, Republic of Korea

关键词: Target selective imaging;    gold nanorods;    SPECT/CT;    photothermal therapy;   

DOI  :  10.3978/.issn.2223-4292.2012.01.03

学科分类：外科医学

来源: AME Publications

【 摘 要 】

The development of theranostic agents with high detection sensitivity and antitumor efficacy at low concentration is achallenging task for target selective imaging and therapy of cancers. In this study, folate-conjugated and radioactive-iodinelabeledgold nanorods (GNRs) were designed and synthesized for target selective SPECT/CT imaging and subsequentthermal ablation of folate-receptor-overexpressing cancers. Both (ortho-pyridyl) disulfide-poly(ethylene glycol)-folate anda short peptide, H2N-Tyr-Asn-Asn-Leu-Ala-Cys-OH, were conjugated on the surface of the GNRs through thiol chemistry.The tyrosine in the peptide sequence was introduced for radioactive-iodine labeling through an iodine-tyrosine interaction.The labeling efficiency of radioactive iodine was more than 99%. Radiochemical stability tests on iodine-125-labeled GNRsin human serum showed that 91% of the iodine-125 remained intact on the GNRs after incubation for 24 h. In vitro andin vivo results in this study confirmed the potential utility of folate-conjugated and iodine-125-labeled GNRs as smarttheranostic agents. This type of platform may also be useful for the targeted SPECT/CT imaging and photothermal therapyof inflammatory diseases such as atherosclerosis and arthritis, in which folate-receptor-overexpressing macrophages playpivotal roles.

【 授权许可】

Unknown