Development, Optimization, Characterization, and Application of Electrochemical Biosensors for Detecting Nickel Ions in Food

Biosensors

Sonia Amariei¹  Gheorghe Gutt¹  Liliana Anchidin-Norocel²  Wesley K. Savage² 

[1] Faculty of Food Engineering, Stefan cel Mare University of Suceava, 720229 Suceava, Romania;Faculty of Medicine and Biological Sciences, Stefan cel Mare University of Suceava, 720229 Suceava, Romania;

关键词: antibodies;    aptamers;    synthetic receptors;    biosensors;    immobilization process;    screen-printed electrodes;   

DOI  :  10.3390/bios11120519

来源: DOAJ

【 摘 要 】

Nickel is naturally present in drinking water and many dietary items, which expose the general population to nickel ingestion. This heavy metal can have a variety of harmful health effects, causing allergies and skin disorders (i.e., dermatitis), lung, cardiovascular, and kidney diseases, and even certain cancers; therefore, nickel detection is important for public health. Recent innovations in the development of biosensors have demonstrated they offer a powerful new approach over conventional analytical techniques for the identification and quantification of user-defined compounds, including heavy metals such as nickel. We optimized five candidate nickel-biosensing receptors, and tested each for efficiency of binding to immobilization elements on screen-printed electrodes (SPEs). We characterized the application of nickel-detecting biosensors with four different cultivated vegetables. We analyzed the efficiency of each nickel-detecting biosensor by potentiostat and atomic absorption spectrometry and compared the results from the sample analytes. We then analyzed the performance characteristics and responses of assembled biosensors, and show they are very effective at measuring nickel ions in food, especially with the urease-alginate biosensor affixed to silver SPEs, measured by cyclic voltammetry (sensitivity—2.1921 µA Mm⁻¹ cm⁻² and LOD—0.005 mg/L). Given the many advantages of biosensors, we describe an optimization pipeline approach to the application of different nickel-binding biosensors for public health, nutrition, and consumer safety, which are very promising.

【 授权许可】

Unknown