Biogenic synthesis, in-vitro cytotoxicity, esterase activity and interaction studies of copper oxide nanoparticles with lysozyme

Journal of Materials Research and Technology

Mehraj ud din Parray¹  Md. Abrar Siddiquee¹  Rajan Patel¹  Maqsood Ahmad Malik²  Majid Rasool Kamli³  Khalid Imtiyaz⁴  M. Moshahid Alam Rizvi⁴  Syed Hassan Mehdi⁴  Hament Kumar Rajor⁵ 

[1] Biophysical Chemistry Laboratory, Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India;Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia;Department of Biological Science, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia;Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India;Department of Chemistry, Ramjas College, University of Delhi, New Delhi, India;

关键词: Biogenic synthesis;    Nanoparticles;    Cytotoxicity;    Lytic activity;    Lysozyme;   

DOI  :  

来源: DOAJ

【 摘 要 】

Herein, we have shown the eco-friendly and low-cost synthesis of biogenic copper oxide nanoparticles (CuONPs) from Delonix regia (D. regia) leaf extract in aqueous medium. The formation of biogenic CuONPs was characterized by ultraviolet (UV)-visible, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) as well as transmission electron microscopy (TEM). Characterization of green synthesized nanoparticles demonstrated a UV–visible peak at 273 nm. Particle size was analysed from TEM monograph in the range of 69–108 nm which validates formation of nanoparticles. The in-vitro anticancer activity was done against A549 (IC50 = 4.70 μg/mL) and MCF7 (IC50 = 3.77 μg/mL) cell line. The biocompatibility assay of these nanoparticles was done on HEK–293 cell line (normal non-cancerous cells). Moreover, the effect of CuONPs on the lytic activity of HEWL was evaluated against Micrococcus lysodeikticus. Further, the interaction of CuONPs with hen egg white lysozyme (HEWL) at different temperature was investigated by steady state fluorescence spectroscopy. UV–visible and far ultra violet-circular dichroism (far UV-CD) spectroscopies were also employed to study the interaction of CuONPs with HEWL. All the results supported each other and show efficient binding between HEWL and CuONPs. The fluorescence emission study revealed that CuONPs quenches the fluorescence of HEWL involving dynamic quenching. The various binding parameters and structural disparity of HEWL in the presence of synthesised CuONPs were also calculated.

【 授权许可】

Unknown