Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead

STAR Protocols

Josie Chrenek¹  Stephanie M. Willerth¹  Kali Scheck²  Rebecca Kirsch² 

[1] Department of Biomedical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada;Department of Mechanical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada;

关键词: Neuroscience;    Stem Cells;    Tissue Engineering;    Biotechnology and bioengineering;    Material sciences;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: 3D bioprinting—a type of additive manufacturing—can create 3D tissue constructs resembling in vivo tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences’ fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be applied to printing 3D tissue models using a variety of cell lines and any chemically crosslinked bioink. These 3D-printed tissue models can be used for applications such as drug screening and disease modeling in vitro.

【 授权许可】

Unknown