Protocol for cryoinjury model in neonatal mice for heart regeneration and repair research

STAR Protocols

Changchun Zeng¹  Rong Chang²  Yanli Zhao³ 

[1] Corresponding author;Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen 518110, China;Department of Cardiovascular Medicine, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, Shenzhen 518110, China;

关键词: Cell Biology;    Model Organisms;    Tissue Engineering;    Biotechnology and bioengineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The variability of animal experimental groups and high maternal cannibalization are two major limitations in cardiac injury models. A cryoinjury model could be an ideal model in heart regeneration and repair research as it can provide reproducible results and the injury size can be scaled. Here, we describe a simple and successful cryoinjury model (rate of mouse survival >90% and rate of maternal cannibalization <5%) for evaluating heart injury in regenerating and non-regenerating mice.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2021).

【 授权许可】

Unknown