A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis

STAR Protocols

Erin N. Morrison¹  Scott C. Farrow²  Mahfuzur R. Shah³  Adam J. Noble³ 

[1] Corresponding author;Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada;Department of Cell Biology, Metabolism and Systems Biology, Noblegen Inc., 2140 East Bank Dr, Peterborough, ON, Canada;

关键词: Cell Biology;    Microbiology;    Model Organisms;    Biotechnology and bioengineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Euglena gracilis is a source of high-value natural products. A major factor affecting consistent production of Euglena biomass is strain stability. Cryopreservation is a leading strategy for cell-line storage that helps ensure process reproducibility. We developed a simple cryopreservation protocol for heterotrophically cultured Euglena that enables the recovery of cells after 1 year with a cell viability of ≅80%. This protocol is suitable for labs interested in the long-term preservation of heterotrophic cultures of Euglena and related species.

【 授权许可】

Unknown