Fucosylated chondroitin sulphate from sea cucumber inhibits high-fat-sucrose diet-induced apoptosis in mouse pancreatic islets via down-regulating mitochondrial signaling pathway

Journal of Functional Foods

Hui Xu¹  Zhojie Li²  Changhu Xue²  Shiwei Hu²  Yuming Wang²  Jingfeng Wang³ 

[1] College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;Corresponding authors. Tel.: [phone].;

关键词: Cucumaria frondosa;    Fucosylated chondroitin sulphate;    Pancreatic islets;    Apoptosis;    Mitochondrial pathway;    Cytochrome c;   

DOI  :  

来源: DOAJ

【 摘 要 】

Hyperglycaemia can induce pancreatic islets apoptosis. We previously found that fucosylated chondroitin sulphate from Cucumaria frondosa (Cf-CHS) exhibited anti-hyperglycaemic effects; however, its effects on pancreatic islets are lacking. This study investigated the effects of Cf-CHS on inhibition pancreatic islets apoptosis in high-fat high-sucrose diet (HFSD)-induced insulin resistant mice for 19 weeks. Results showed that Cf-CHS significantly repaired HFSD-injured pancreatic islets, decreased blood glucose, insulin, TNF-α levels, and increased adiponectin level. Cf-CHS significantly reduced Bid, Bax, cytochrome c, caspase 9, and caspase 3 mRNA expressions, and increased Bcl-2 and Bcl-xL mRNA expressions. Cf-CHS also caused significant down-regulation of t-Bid, Bax, cytochrome c in cytoplasm, caspase 9, and cleaved-caspase 3 proteins, and up-regulation of Bcl-2 and Bcl-xL proteins. Furthermore, Cf-CHS enhanced the effects of rosiglitazone (RSG). These indicate that Cf-CHS inhibits pancreatic islets apoptosis via inhibition mitochondrial pathway. These findings may provide a dietary intervention hyperglycaemia-induced pancreatic islets apoptosis.

【 授权许可】

Unknown