Frontiers in Immunology | |
MHC class I cross-presentation by Dendritic Cells counteracts viral immune evasion | |
Kenneth M Murphy1  Ulrich Helmut Koszinowski2  Christine eRied2  Caroline Andree Bernhard2  Katrin eNopora2  Thomas eBrocker2  Alejandro A Castello3  Peggy eMarconi4  | |
[1] Howard Hughes Medical Institute;Ludwig-Maximilians-University Munich;Universidad Nacional de Quilmes;University of Ferrara; | |
关键词: Antigen Presentation; Cross-Priming; Dendritic Cells; Immune Evasion; Virus infection; | |
DOI : 10.3389/fimmu.2012.00348 | |
来源: DOAJ |
【 摘 要 】
DCs very potently activate CD8+ T cells specific for viral peptides bound to MHC class I molecules. However, many viruses have evolved immune evasion mechanisms, which inactivate infected DCs and might reduce priming of T cells. Then MHC class I cross-presentation of exogenous viral Ag by non-infected DCs may become crucial to assure CD8+ T cell responses. Although many vital functions of infected DCs are inhibited in vitro by many different viruses, the contributions of cross-presentation to T cell immunity when confronted with viral immune inactivation in vivo has not been demonstrated up to now, and remains controversial. Here we show that priming of Herpes Simplex Virus (HSV)-, but not murine cytomegalovirus (mCMV)-specificCD8+ T cells was severely reduced in mice with a DC-specific cross-presentation deficiency. In contrast, while CD8+ T cell responses to mutant HSV, which lacks crucial inhibitory genes, also depended on CD8+ DCs, they were independent of cross-presentation. Therefore HSV-specific CTL-responses entirely depend on the CD8+ DC-subset, which present via direct or cross-presentation mechanisms depending on the immune-evasion equipment of virus. Our data establish the contribution of cross-presentation to counteract viral immune evasion mechanisms in some, but not all viruses.
【 授权许可】
Unknown