Phase Mask-Based Multimodal Superresolution Microscopy

Photonics

Jeremiah W. Woodcock¹  Stephan J. Stranick¹  Jeffrey W. Gilman¹  Ryan Beams¹ 

[1] Material Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Drive, MD 20899, USA;

关键词: microscopy;    superresolution;    nonlinear microscopy;   

DOI  :  10.3390/photonics4030039

来源: DOAJ

【 摘 要 】

We demonstrate a multimodal superresolution microscopy technique based on a phase masked excitation beam in combination with spatially filtered detection. The theoretical foundation for calculating the focus from a non-paraxial beam with an arbitrary azimuthally symmetric phase mask is presented for linear and two-photon excitation processes as well as the theoretical resolution limitations. Experimentally this technique is demonstrated using two-photon luminescence from 80 nm gold particle as well as two-photon fluorescence lifetime imaging of fluorescent polystyrene beads. Finally to illustrate the versatility of this technique we acquire two-photon fluorescence lifetime, two-photon luminescence, and second harmonic images of a mixture of fluorescent molecules and 80 nm gold particles with <120 nm resolution ( λ /7). Since this approach exclusively relies on engineering the excitation and collection volumes, it is suitable for a wide range of scanning-based microscopies.

【 授权许可】

Unknown