Application of quantitative second-harmonic generation imaging to dynamic systems

nonlinear microscopy;Second harmonic generation;Image analysis

Kabir, Mohammad ; Toussaint ; Kimani C.

关键词: nonlinear microscopy;    Second harmonic generation;    Image analysis;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/49574/Mohammad_Kabir.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

The purpose of this thesis is to report the development of a quantitative second harmonic generation technique that quantifies the 2 dimensional spatial orientation of collagen fiber samples under dynamic conditions. The technique is demonstrated both for a well aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, the applicability of this technique is confirmed for various window sizes (pixel sizes) as well as with using a gridded overlay map that allows for the correlations of fiber orientations within a given image. Additionally, we adapted a graphics processing unit (GPU) to the image analysis with an aim to provide a reduction in image processing time. There, we demonstrate the temporal advantage of the GPU-based approach by computing the number of frames analyzed per second for SHG image videos showing varying fiber orientations. In comparison to a CPU-based image analysis technique, the GPU-based system results in ~ 10x improvement in computational time. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis. The adaptation of a GPU to the analysis also introduces this approach to other quantitative, nonlinear imaging techniques.