Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar

Wyman, Charles¹  Balan, Venkatech²  Dale, Bruce E.²  Elander, Richard³ 

[1]Univ. of California, Riverside, CA (United States)

[2]Michigan State Univ., East Lansing, MI (United States)

[3]National Renewable E

关键词: hydrolysis;    microscopy;    pretreatment;    switchgrass;    yields;   

DOI  :  10.2172/1261642
RP-ID  :  DOE-UCR--17102
PID  :  OSTI ID: 1261642

学科分类：化学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

Dilute sulfuric acid (DA), sulfur dioxide (SO2), liquid hot water (LHW), soaking in aqueous ammonia (SAA), ammonia fiber expansion (AFEX), and lime pretreatments were applied to Alamo, Dacotah, and Shawnee switchgrass. Application of the same analytical methods and material balance approaches facil-itated meaningful comparisons of glucose and xylose yields from combined pretreatment and enzymatic hydrolysis. Use of a common supply of cellulase, beta-glucosidase, and xylanase also eased comparisons. All pretreatments enhanced sugar recovery from pretreatment and subsequent enzymatic hydrolysis substantially compared to untreated switchgrass. Adding beta-glucosidase was effective early in enzy-matic hydrolysis while cellobiose levels were high but had limited effect on longer term yields at the enzyme loadings applied. Adding xylanase improved yields most for higher pH pretreatments where more xylan was left in the solids. Harvest time had more impact on performance than switchgrass variety, and microscopy showed changes in different features could impact performance by different pretreatments.