In Vivo Production of Monoclonal Antibodies by Gene Transfer via Electroporation Protects against Lethal Influenza and Ebola Infections

Molecular Therapy: Methods & Clinical Development

Yang Luo¹  Chasity D. Andrews¹  David D. Ho¹  Ming Sun¹  Jian Yu¹  Neal N. Padte¹  Yaoxing Huang¹  Pamela J. Glass²  Arthur J. Goff² 

[1] Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA;US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA;

关键词: plasmid;    DNA-based antibody gene transfer;    electroporation;    infectious disease;    influenza;    Ebola;   

DOI  :  10.1016/j.omtm.2017.09.003

来源: DOAJ

【 摘 要 】

Monoclonal antibodies (mAbs) have wide clinical utility, but global access is limited by high costs and impracticalities associated with repeated passive administration. Here, we describe an optimized electroporation-based DNA gene transfer platform technology that can be utilized for production of functional mAbs in vivo, with the potential to reduce costs and administration burdens. We demonstrate that multiple mAbs can be simultaneously expressed at protective concentrations for a protracted period of time using DNA doses and electroporation conditions that are feasible clinically. The expressed mAbs could also protect mice against lethal influenza or Ebola virus challenges. Our findings suggest that this DNA gene transfer platform technology could be a game-changing advance that expands access to effective mAb therapeutics globally.

【 授权许可】

Unknown