Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers

Nanoscale Research Letters

Jinyang Zhang¹  Le Jing¹  Qiang Chen¹  Xueshan Xia¹  Jie Li¹  Binghui Wang¹  Yuzhu Song¹  Qinqin Han¹ 

[1] Engineering Research Center for Molecular Diagnosis, Faculty of Life Science and Technology, Kunming University of Science and Technology;

关键词: Aptamer;    AuNPs;    Lateral flow strip;    Detection;    Rongalite;   

DOI  :  10.1186/s11671-018-2709-9

来源: DOAJ

【 摘 要 】

Abstract A sandwich lateral flow strip assay (LFSA) using a couple of aptamers functionalized with gold nanoparticles (AuNPs) was designed to assess the presence of rongalite in agrifood products. More specifically, a biotin-labeled primary A09 aptamer immobilized on a streptavidin-coated membrane and a secondary B09 aptamer conjugated with AuNPs were developed as capturing and signaling probes, respectively. This system allows the successful and direct detection of rongalite in food samples with concentrations as low as 1 μg/mL, simply by observing the color change of LFSA control and test line.

【 授权许可】

Unknown