A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

Food and Agricultural Immunology

Fangqin Pang¹  Ruifeng Luo¹  Hongmin Li¹  Xiayu Peng²  Lichao Kang³  Fengxia Sun³  Xiaoling Luo³ 

[1] Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, People’s Republic of Chin;State Key Laboratory of Sheep Genetic Improvement & Healthy Breeding, Shihezi, People’s Republic of Chin;State Key Laboratory of Sheep Genetic Improvement & Healthy Breeding, Shihezi, People’s Republic of Chin;Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, People’s Republic of Chin;

关键词: Lateral flow strip;    signal amplification;    BPA;    food safety;   

DOI  :  10.1080/09540105.2017.1365822

来源: publisher

【 摘 要 】

A signal-enhanced lateral flow strip biosensor has been constructed for ultrasensitive and on-site visual detection of bisphenol A (BPA). The signal amplification principle is based on the capacity of binding a large number of antibody-assembled gold nanoparticle (AuNP–antibody) conjugates of the poly amidoamine (PAMAM), which bears a multitude of amino groups. This signal-enhanced method could achieve BPA detection at 10 ppb based on naked eye visual observation, which represents an at least 50-fold improvement of the sensitivity of a traditional method without PAMAM. Moreover, good linearity was observed in the range from 2 to 100 ppb after quantitative analysis using the ImageJ software. This signal amplification method could be adopted as a potential generous technique in different fields.

【 授权许可】

CC BY