Food and Agricultural Immunology | |
A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A | |
Fangqin Pang1  Ruifeng Luo1  Hongmin Li1  Xiayu Peng2  Lichao Kang3  Fengxia Sun3  Xiaoling Luo3  | |
[1] Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, People’s Republic of Chin;State Key Laboratory of Sheep Genetic Improvement & Healthy Breeding, Shihezi, People’s Republic of Chin;State Key Laboratory of Sheep Genetic Improvement & Healthy Breeding, Shihezi, People’s Republic of Chin;Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, People’s Republic of Chin; | |
关键词: Lateral flow strip; signal amplification; BPA; food safety; | |
DOI : 10.1080/09540105.2017.1365822 | |
来源: publisher | |
【 摘 要 】
A signal-enhanced lateral flow strip biosensor has been constructed for ultrasensitive and on-site visual detection of bisphenol A (BPA). The signal amplification principle is based on the capacity of binding a large number of antibody-assembled gold nanoparticle (AuNP–antibody) conjugates of the poly amidoamine (PAMAM), which bears a multitude of amino groups. This signal-enhanced method could achieve BPA detection at 10 ppb based on naked eye visual observation, which represents an at least 50-fold improvement of the sensitivity of a traditional method without PAMAM. Moreover, good linearity was observed in the range from 2 to 100 ppb after quantitative analysis using the ImageJ software. This signal amplification method could be adopted as a potential generous technique in different fields.
【 授权许可】
CC BY
【 预 览 】
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RO202004238978551ZK.pdf | 1768KB | download |