Screening of different thermal receipt papers for their potential endocrine activity

Thermal paper;receipt paper;BPA;alternatives;BPA free;whole extraction;thyroid hormone;sex hormone;363.7

보건대학원 환경보건학과 ;

University:서울대학교 보건대학원

关键词: Thermal paper;    receipt paper;    BPA;    alternatives;    BPA free;    whole extraction;    thyroid hormone;    sex hormone;    363.7;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/137707/1/000000146024.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

therefore, structural or functional alternatives to BPA have been replaced and manufactured. However, we have limited information on their potential adverse effects. In this study, we obtained three kinds of thermal receipt papers, i.e., BPA, BPA free, non-bisphenol thermal receipt papers, and screened for their potential endocrine effects. The extracts of three receipt papers were performed on GH3 and H295R cell lines to investigate their possible disruptive effects in thyroid or sex hormone systems. The amount of color developer on thermal receipt paper is equivalent to 1 % of total receipt paper weight. Both cell lines were exposed to the extracts with 0, 0.0015, 0.005, 0.015, 0.05, or 0.15 mg developer/g receipt paper in 0.1 % ethanol of cell medium. In GH3 cells, BPA and BPA free thermal receipt extracts reduced thyroid stimulating hormone (Tsh) gene expression level significantly. However, other thyroid hormone related genes such as Trα, Trβ, Dio1, and Dio2 did not showed any down- or up-regulations after the exposure. Those two extracts also reduced transcriptional levels in follicular stimulating hormone (Fsh), estrogen receptor α (Erα), and β (Erβ). Non-bisphenol thermal receipt paper extract did not show any alteration in both thyroid and gonad axis related genes. In H295R cell lines, all three thermal receipt paper extracts increased in production of 17β-estradiol at their highest concentrations (0.05 and 0.15 mg/g receipt paper). However, they did not lead to any changes in testosterone level and related gene transcription levels. In this study, all three investigated thermal receipt papers showed endocrine disruption potential on either thyroid or sex hormone system. The results of this study shows that BPA free and non-bisphenol thermal receipt papers may not be safer alternatives to BPA thermal receipt paper. This study showed possible toxic effects from all three extracts of thermal receipt papers. Further studies should follow to evaluate the toxicities of BPA free receipt paper in vivo to develop better substituting chemicals for color development process in receipt paper.