Rapid antibody testing for SARS-CoV-2 vaccine response in pediatric healthcare workers

International Journal of Infectious Diseases

Aprille Tongol¹  Diane Nugent²  Theodore W. Heyming³  Kellie Bacon⁴  Chloe Knudsen-Robbins⁴  Bryan Lara⁵  Terence Sanger⁵  Janet Hoang⁵  John Schomberg⁶ 

[1] Corresponding author: Theodore Heyming, Children's Hospital of Orange County, 1201 W La Veta Ave, Orange, CA 92868, USA. Tel: [phone]. Fax: [phone];Department of Emergency Medicine, University of California, Irvine, California, USA;Department of Emergency Medicine, Children's Hospital of Orange County, Orange, California, USA;Department of Hematology, Children's Hospital of Orange County, Orange, California, USA;Research Institute, Children's Hospital of Orange County, Orange, California, USA;University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA;

关键词: COVID-19;    SARS-CoV-2;    Pediatric healthcare workers;    SARS-CoV-2 vaccine;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: The durability of the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination remains unknown. The objective of this study was to evaluate a rapid SARS-CoV-2 IgM/IgG antibody detection kit as a qualitative screen for the humoral response to vaccination.Methods: Study participants (n = 125) included pediatric healthcare workers (HCWs) who had received two doses of BNT162b2 or mRNA-1273. Participants were tested on study entry (March 12, 2021 to April 9, 2021). The mean number of days post second dose was 22 (range 17–36). Participants were tested for IgM/IgG antibodies to the SARS-CoV-2 spike protein with the RightSign COVID-19 IgG/IgM Rapid Test Cassette. ELISA/competitive inhibition ELISA (CI-ELISA) were subsequently run to assess for the neutralization effect and SARS-CoV-2 anti-nucleocapsid IgM/IgG antibodies.Results: Overall, 98.4% of participants were IgG-positive and 0.8% were IgM-positive on rapid RightSign testing. Of those with IgG-positive results, 100% were anti-spike protein IgG-positive on CI-ELISA; none of those who tested IgG-negative via the rapid test were IgG-positive on CI-ELISA. All HCWs who tested RightSign positive demonstrated neutralizing capability on CI-ELISA. Overall, 1.6% demonstrated anti-nucleocapsid IgM antibodies and 5.6% demonstrated anti-nucleocapsid IgG antibodies.Conclusions: The strong agreement between the rapid RightSign IgG results and confirmatory CI-ELISA testing suggests that this test may be used to assess for positive, and neutralizing, antibody responses to SARS-CoV-2 mRNA vaccination.

【 授权许可】

Unknown