Frontiers in Endocrinology | |
Differential Expression of Keratinocyte-Derived Extracellular Vesicle Mirnas Discriminate Exosomes From Apoptotic Bodies and Microvesicles | |
Carlos Salomon1  James A. Broadbent3  Tony J. Parker3  Dominic Guanzon4  David I. Leavesley4  Uyen T.T. Than6  | |
[1] Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, University of Concepción, Concepción, Chile;Exosome Biology Laboratory, Centre for Clinical Diagnostics, University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, The University of Queensland, Brisbane, QLD, Australia;Faculty of Health, School of Biomedical Science, Queensland University of Technology, Brisbane, QLD, Australia;Institute of Medical Biology–Agency for Science, Technology and Research, Singapore, Singapore;Tissue Repair and Translational Physiology Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, Australia;Vinmec Research Institute of Stem Cell and Gene Technology, Vinmec International Hospital, Ha Noi, Vietnam;Wound Management Innovation Cooperative Research Centre, West End, QLD, Australia; | |
关键词: extracellular vesicles; apoptotic body; microvesicle; exosome; microRNA; keratinocytes; | |
DOI : 10.3389/fendo.2018.00535 | |
来源: DOAJ |
【 摘 要 】
Extracellular vesicles (EVs) are mammalian cell-derived nano-scale structures enclosed by a lipid bilayer that were previously considered to be cell debris with little biological value. However, EVs are now recognized to possess biological function, acting as a packaging, transport and delivery mechanisms by which functional molecules (i.e., miRNAs) can be transferred to target cells over some distance. To examine the miRNA from keratinocyte-derived EVs, we isolated three distinct populations of EVs from both HaCaT and primary human keratinocytes (PKCs) and characterized their biophysical, biochemical and functional features by using microscopy, immunoblotting, nanoparticle tracking, and next generation sequencing. We identified 1,048; 906; and 704 miRNAs, respectively, in apoptotic bodies (APs), microvesicles (MVs) and exosomes (EXs) released from HaCaT, and 608; 506; and 622 miRNAs in APs, MVs and EXs released from PKCs. In which, there were 623 and 437 identified miRNAs common to three HaCaT-derived EVs and PKC-derived EVs, respectively. In addition, we found hundreds of exosomal miRNAs that were previously un-reported. Differences in the abundance levels of the identified EV miRNAs could discriminate between the three EV populations. These data contribute substantially to knowledge within the EV-identified miRNA database, especially with regard to keratinocyte-derived EV miRNA content.
【 授权许可】
Unknown