Actin and Actin-Associated Proteins in Extracellular Vesicles Shed by Osteoclasts

International Journal of Molecular Sciences

LorrainePerciliano de Faria¹  WellingtonJ. Rody²  L.Shannon Holliday³ 

[1] Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo 01000, Brazil;Department of Orthodontics and Pediatric Dentistry, Stony Brook University School of Dental Medicine, Stony Brook, NY 11794, USA;Department of Orthodontics, College of Dentistry, University of Florida, Gainesville, FL 32610, USA;

关键词: exosome;    microvesicle;    microfilament;    integrins;    bone remodeling;    myosins;    actin-related protein;    proteomics;    extracellular vesicles;   

DOI  :  10.3390/ijms21010158

来源: DOAJ

【 摘 要 】

Extracellular vesicles (EVs) are shed by all eukaryotic cells and have emerged as important intercellular regulators. EVs released by osteoclasts were recently identified as important coupling factors in bone remodeling. They are shed as osteoclasts resorb bone and stimulate osteoblasts to form bone to replace the bone resorbed. We reported the proteomic content of osteoclast EVs with data from two-dimensional, high resolution liquid chromatography/mass spectrometry. In this article, we examine in detail the actin and actin-associated proteins found in osteoclast EVs. Like EVs from other cell types, actin and various actin-associated proteins were abundant. These include components of the polymerization machinery, myosin mechanoenzymes, proteins that stabilize or depolymerize microfilaments, and actin-associated proteins that are involved in regulating integrins. The selective incorporation of actin-associated proteins into osteoclast EVs suggests that they have roles in the formation of EVs and/or the regulatory signaling functions of the EVs. Regulating integrins so that they bind extracellular matrix tightly, in order to attach EVs to the extracellular matrix at specific locations in organs and tissues, is one potential active role for actin-associated proteins in EVs.

【 授权许可】

Unknown