Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end

Genes and Environment

Toshiyuki Saito¹  Hisaka Kurita²  Seiichiroh Ohsako²  Toshiki Aiba² 

[1] Department of Radiation Effects Research, National Institutes for Quantum and Radiological Science and Technology;Laboratory of Environmental Health Science, Center for Disease Biology and Integrative Medicine, The University of Tokyo;

关键词: Restriction enzyme;    Labeling;    5′-Methylcytosine;    Methylome;   

DOI  :  10.1186/s41021-017-0089-5

来源: DOAJ

【 摘 要 】

Abstract Environmental factors stimulate alteration of DNA methylation level. Investigation of the genome-wide DNA methylation status is important for environmental health studies. We here designed a genomic DNA amplification and labeling protocol using a methylation-sensitive restriction enzyme HinP1 I. This method can specifically amplify genomic DNA fragments possessing methyl-CpG at the end. The fragments are a relatively short size and dominantly located on CpG-islands. By using the samples prepared by this method, a dioxin-induced change in the methylation level of the mouse Cyp1a1 promoter was successfully evaluated using oligonucleotide probes covalently bound onto a glass plate. The method developed in this paper would be useful for other genome-wide analysis platforms for the large scale epigenome-wide association studies (EWAS) including human epidemiological samples.

【 授权许可】

Unknown