Analysis of the Euglena gracilis chloroplast genome

FEBS Letters

Stutz, Erhard¹  Keller, Mario¹  Rutti, Bernard¹ 

[1] Laboratoire de Biochimie, Université de Neuchâtel, Ch. de Chantemerle 18, CH-2000 Neuchâtel, Switzerland

关键词: Euglena gracilis;    Chloroplast genome;    Thylakoid membrane;    Protein 32 00 M r;    Restriction enzyme;    DNA fragment Eco-I;   

DOI  :  10.1016/0014-5793(82)81088-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The DNA fragment Eco-I (4700 kilobasepairs) from the circular chloroplast genome of Euglena gracilis is shown to be transcribed in etioplasts at all stages of light-induced plastid development and in fully differentiated chloroplasts. Major stable transcription products are mRNAs of 14S and 17S. Using a rabbit reticulocyte lysate translation system we can show that the fragment Eco-I selects a mRNA which directs the synthesis of a M _r 32 000–33 000 polypeptide. Eco-I also hybridizes with a 330 basepair DNA probe cut from within the spinach chloroplast gene encoding the M _r 32 000 thylakoid membrane protein of the photosystem II reaction center. We conclude that the fragment Eco-I carries the corresponding Euglena gracilis chloroplast gene.

【 授权许可】

Unknown