Purification and characterization of endonucleases DraII and III from Deinococcus radiophilus

FEBS Letters

de Waard, A.¹  Neele, A.C.¹  de Wit, C.M.¹  Dekker, B.M.M.¹ 

[1] Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands

关键词: Restriction enzyme;    Nucleotide sequence;    Ad;    adenovirus;    FPLC;    fast protein liquid chromatography;    Pu;    purine;    Py;    pyrimidine;    mec;    gene for deoxycytidylate methyl transferase;   

DOI  :  10.1016/0014-5793(85)81074-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deinococcus radiophilus strain ATCC 27603 contains, apart from endonuclease DraI [1], two additional sequence-specific endonucleases. These enzymes, designated DraII and DraIII, recognize nucleotide sequences with novel specificities, PuG↓GNCCPy and CACNN↓GTG, respectively.

【 授权许可】

Unknown