Discrimination between Leave of Apocynum venetum and Its Adulterant, A. pictum Based on AntioxidantAssay and Chemical Profiles Combined withMultivariate Statistical Analysis

Antioxidants

Ching-Ching Lau¹  Yam-Fung Ng¹  Shun-Wan Chan²  Daniel Kam-Wah Mok²  Chi-On Chan²  Si-Bao Chen²  Li-Jia Xu³ 

[1] Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China;State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Shenzhen 518057, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China;

关键词: Apocynum venetum L.;    Apocynum pictum Schrenk;    HPLC fingerprints;    free radical scavenging capacity;   

DOI  :  10.3390/antiox4020359

来源: DOAJ

【 摘 要 】

An integrated approach including chemical and biological assessments was developed to investigate the differences between Apocynum venetum L. (AV) and its adulterant, Apocynum pictum Schrenk (AP). Ten flavonoids were tentatively identified by ultra-visible and mass spectra data. The chemical component, hyperoside, was identified as a critical parameter for discrimination of two species from the results of principal component analysis (PCA) and quantitative analysis. The anti-oxidative power of the herbal extracts were determined using 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay and H2O2-induced cell damage on LO2 cells. The results of the biological assays suggested that the chemical differences between AV and AP do lead to difference in activity and AV is demonstrated to have higher anti-oxidant activity.

【 授权许可】

Unknown