Study on HPLC Fingerprint of Clematis manshurica Rupr from Different Areas

2019 3rd International Workshop on Renewable Energy and Development

能源学;生态环境科学

Kong, Juan^1 ; Huang, Xulong^1 ; Yang, Xiaosong^1 ; Zhang, Lingling^1 ; Li, Xiaofen^1 ; Zeng, Qian^1 ; Wu, Hongmei^1 ; Wang, Xiangpei^1

Department of Pharmacognosy, Guiyang University of Chinese Medicine, Nanming District 50, Guizhou Province, Guiyang City, Guiyang

550002, China^1

关键词: Acetonitril;    Analytical method;    Chromatographic column;    Column temperature;    Common peak;    HPLC fingerprints;    Mobile phase;    Similarity evaluation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/267/6/062053/pdf
DOI  :  10.1088/1755-1315/267/6/062053

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Objective: To establish HPLC fingerprint of Clematis manshurica Rupr from different areas. Method: 10 batches of Clematis manshurica Rupr was analyzed by RP-HPLC that the methods were used Odyssil-C18 chromatographic column, with the mobile phase of acetonitril-0.1% phosphoric acid solution, the flow rate was 0.8mL/min, the wavelength was 210 nm, and column temperature was 30C. And the similarity was evaluated by similarity evaluation software. Result: The HPLC-UV fingerprint of Clematis manshurica Rupr was established, and 20 common peaks were determined, the similarity was between 0.974-0.823, indicating that the differences of Clematis manshurica Rupr from different regions were significant. Conclusion: The analytical method has good separation degree and feasibility, and it can provide a reliable basis for the quality control of Clematis manshurica Rupr.