2019 3rd International Workshop on Renewable Energy and Development | |
Study on HPLC Fingerprint of Clematis manshurica Rupr from Different Areas | |
能源学;生态环境科学 | |
Kong, Juan^1 ; Huang, Xulong^1 ; Yang, Xiaosong^1 ; Zhang, Lingling^1 ; Li, Xiaofen^1 ; Zeng, Qian^1 ; Wu, Hongmei^1 ; Wang, Xiangpei^1 | |
Department of Pharmacognosy, Guiyang University of Chinese Medicine, Nanming District 50, Guizhou Province, Guiyang City, Guiyang | |
550002, China^1 | |
关键词: Acetonitril; Analytical method; Chromatographic column; Column temperature; Common peak; HPLC fingerprints; Mobile phase; Similarity evaluation; | |
Others : https://iopscience.iop.org/article/10.1088/1755-1315/267/6/062053/pdf DOI : 10.1088/1755-1315/267/6/062053 |
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学科分类:环境科学(综合) | |
来源: IOP | |
【 摘 要 】
Objective: To establish HPLC fingerprint of Clematis manshurica Rupr from different areas. Method: 10 batches of Clematis manshurica Rupr was analyzed by RP-HPLC that the methods were used Odyssil-C18 chromatographic column, with the mobile phase of acetonitril-0.1% phosphoric acid solution, the flow rate was 0.8mL/min, the wavelength was 210 nm, and column temperature was 30C. And the similarity was evaluated by similarity evaluation software. Result: The HPLC-UV fingerprint of Clematis manshurica Rupr was established, and 20 common peaks were determined, the similarity was between 0.974-0.823, indicating that the differences of Clematis manshurica Rupr from different regions were significant. Conclusion: The analytical method has good separation degree and feasibility, and it can provide a reliable basis for the quality control of Clematis manshurica Rupr.
【 预 览 】
Files | Size | Format | View |
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Study on HPLC Fingerprint of Clematis manshurica Rupr from Different Areas | 470KB | download |