14th International Conference on Science, Engineering and Technology | |
Development and validation of RP-UHPLC procedure for estimation of 5-amino salicyclic acid in 5-amino salicyclic acid rectal suppositories | |
自然科学;工业技术 | |
Balaji, Jayagopal^1 ; Shivashankar, Murugesh^1 | |
Department of Chemistry, School of Advanced Sciences, VIT University, Vellore-14, Tamil Nadu, India^1 | |
关键词: Coefficient of variation; Column temperature; Concentration ranges; Injection volume; Percentage recovery; Phosphate buffers; Salicyclic acids; Ultra performance liquid chromatography; | |
Others : https://iopscience.iop.org/article/10.1088/1757-899X/263/2/022025/pdf DOI : 10.1088/1757-899X/263/2/022025 |
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来源: IOP | |
【 摘 要 】
The present study describes a simple and robust reverse phase ultra performance liquid chromatography (RP-UPLC) method for the quantification of 5-amino salicyclic acid in 5-amino salicyclic acid rectal capsules. Successful separation of Mesalamine peak from excipient peaks and diluent were achieved on a Acquity C8 (50 × 2.1 mm, 1.7 μm) and UV detector at 254 nm, 0.3 mL/min as a flow rate, and 3 μL as an injection volume. For the RP-UPLC method, phosphate buffer and methanol was used as mobile phases at ratio of 83:17 and the column temperature was 25 °C. Percentage recovery obtained in the range of 98.7-99.7 % and the method is linear for Mesalamine for specified concentration range with coefficient of variation (r) not less than 0.99. The proposed RP-UPLC method was found to be specific, linear, precise, accurate and robust.
【 预 览 】
Files | Size | Format | View |
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Development and validation of RP-UHPLC procedure for estimation of 5-amino salicyclic acid in 5-amino salicyclic acid rectal suppositories | 861KB | download |