Determination of Echinocystic Acid in Extract of Spina Gleditsiae by HPLC

2018 International Conference on Civil and Hydraulic Engineering

土木建筑工程;水利工程

Liu, Xiaojuan^1 ; Cao, Meiyu^1

Jiangsu Provincial Xuzhou Pharmaceutical Vocational College, Xuzhou, China^1

关键词: Average recovery;    Chromatographic conditions;    Column temperature;    Concentration ranges;    Detection wavelengths;    Kromasil C18;    Linear relationships;    Mobile phase;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/189/2/022012/pdf
DOI  :  10.1088/1755-1315/189/2/022012

学科分类：土木及结构工程学

来源: IOP

【 摘 要 】

A high performance liquid chromatography (HPLC) method for the determination of echinocystic acid in spina gleditsiae was established. Chromatographic conditions were as follows: The separation was performed on Dikma Kromasil C18 column (250 mm 4.6 mm, 5 μm) with column temperature kept at 30 °C. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (45:55) at a flow rate of 1.0 mL/min with detection wavelength set at 215 nm. The sample input was 10 μL. The results showed that echinocystic acid had a good linear relationship in the concentration range of 3.16-63.4 μg/mL. The average recovery was 98.21% and RSD was 0.93% (n = 9). The established method was proved to sensitive, accurate, simple, repeatable and applicable for the quality control of the extracts of spina gleditsiae.