Carp Edema Virus Infection Is Associated With Severe Metabolic Disturbance in Fish

Frontiers in Veterinary Science

Jana Blahova¹  Ivana Mikulikova²  Małgorzata Bednarska³  Hana Minarova⁴  Lubomir Pojezdal⁴  Hana Bandouchova⁵  Jiri Pikula⁵  Ivana Papezikova⁵  Miroslava Palikova⁵  Jan Mares⁵ 

[1] Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czechia;Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czechia;Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland;Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czechia;Department of Zoology, Fisheries, Hydrobiology and Apiculture, Mendel University in Brno, Brno, Czechia;

关键词: emerging viral diseases;    fish;    pathophysiology;    electrolyte and acid-base imbalance;    hypotonic dehydration;    endogenous hyperammonaemia;   

DOI  :  10.3389/fvets.2021.679970

来源: DOAJ

【 摘 要 】

Significant mortalities associated with emerging viral diseases are challenging the economy of common carp aquaculture. As such, there is an increased need to disentangle how infected fish cope with progressive disease pathology and lose the ability for homeostatic maintenance of key physiological parameters. A natural carp edema virus (CEV) infection outbreak at a carp fish farm provided an opportunity to examine diseased and healthy carp in the same storage pond, thereby contributing to our better understanding of CEV disease pathophysiology. The disease status of fish was determined using PCR-based virus identification combined with analysis of gill pathology. Compared with healthy control carp, the blood chemistry profile of CEV-infected fish revealed major disruptions in electrolyte and acid-base balance (i.e., hyponatraemia, hypochloraemia, hyperphosphatemia, elevated pH, base excess, and anion gap and decreased partial dissolved carbon dioxide). In addition, we recorded hyperproteinaemia, hyperalbuminaemia, hypotonic dehydration, endogenous hyperammonaemia, and decreased lactate along with increased creatinine, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. Red blood cell associated hematology variables were also elevated. The multivariate pattern of responses for blood chemistry variables (driven by sodium, pH, partial dissolved carbon dioxide, ammonia, and albumin in the principal component analysis) clearly discriminated between CEV-infected and control carp. To conclude, we show that CEV infection in carp exerts complex adverse effects and results in severe metabolic disturbance due to the impaired gill respiratory and excretory functioning.

【 授权许可】

Unknown