Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens

Viruses

Antonio Zapatero¹  Jesús Canora¹  FranciscoJavier Martínez-Peromingo¹  Pablo Barreiro²  Marta Pérez-Abeledo²  Belén Ramos²  FranciscoJavier Candel²  JoséManuel Viñuela-Prieto²  JuanCarlos Sanz²  Jesús San Román²  María del Mar Carretero² 

[1] Council of Public Health, Community of Madrid, Calle O’Donnell, 50, 28009 Madrid, Spain;Public Health Regional Laboratory, Hospital Isabel Zendal, Av. Manuel Fraga Iribarne, 2, 28055 Madrid, Spain;

关键词: SARS-CoV-2;    COVID-19;    serology;    IgM;    spike;    nucleocapsid;   

DOI  :  10.3390/v13050874

来源: DOAJ

【 摘 要 】

The virological meaning of the different patterns of serology in COVID-19 has been little examined in clinical settings. Asymptomatic subjects with IgM-spike (S) and IgG-nucleocapsid (N) determinations by chemiluminescence were studied for SARS-CoV-2 shedding in respiratory secretions by transcription-mediated amplification (TMA). In subjects showing IgM-S positive and IgG-N negative, IgG-S was determined by lateral flow assay. A total of 712 individuals were tested: 30.0% presented IgM-S(+)/IgG-N(−), 25.8% had IgM-S(+)/IgG-N(+) and 44.2% had IgM-S(−)/IgG-N(+); the proportion with TMA(+) were comparable in these three groups: 12.1, 8.7 and 10.5%, respectively. In individuals with IgM-S(+)/IgG-N(−), IgG-S(+) was detected in 66.5%. The frequency of IgM-S(+)/IgG-S(−) in the total population was 10.0%, of whom 24.1% had TMA(+); the chances for TMA(+) in subjects with an IgM-S(+) alone pattern were 2.4%. Targeting of the same SARS-CoV-2 antigen seems to be better for the characterization of IgM/IgG patterns of response. IgM-S(+) alone reactivity is rare, and a small proportion is associated with viral shedding.

【 授权许可】

Unknown