Microbial Contamination of Photographic and Cinematographic Materials in Archival Funds in the Czech Republic

Microorganisms

Dominika Kalisova¹  Sabina Purkrtova¹  Jana Kadava¹  Tereza Nesporova¹  Hana Sykorova¹  Katerina Demnerova¹  Dana Savicka¹  Nikola Kovacova¹  Martina Novakova²  Barbora Masek Benetkova²  Lucie Koukalova²  Michal Durovic²  Blanka Hnulikova³  Stepanka Boryskova³ 

[1] Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, 5 Technická, 166 28 Prague, Czech Republic;Department of Chemical Technology of Monument Conservation, Faculty of Chemical Technology, University of Chemistry and Technology Prague, 5 Technická, 166 28 Prague, Czech Republic;Preservation Department, National Archives Czech Republic, 2257/4 Archivní, 149 00 Prague, Czech Republic;

关键词: photographic materials;    cinematographic materials;    archival funds;    Czech Republic;    microbial contamination;    fungal contamination;   

DOI  :  10.3390/microorganisms10010155

来源: DOAJ

【 摘 要 】

In this study we investigated the microbial contamination of 126 samples of photographic and cinematographic materials from 10 archival funds in the Czech Republic. Microorganisms were isolated from the light-sensitive layer by swabbing it with a polyurethane sponge. Microbial isolates were identified by MALDI-TOF MS (bacteria) or by phenotype testing and microscopy (fungi). Bacterial contamination was more abundant and more diverse than fungal contamination, and both were significantly associated with archives. The most frequently isolated fungal genera were Cladosporium, Eurotium, Penicillium, Aspergillus and Alternaria. The most frequently isolated bacteria were Gram-positive genera such as Staphylococcus, Micrococcus, Kocuria, Streptococcus and Bacillus. This bacterial and fungal diversity suggests that air is the main vehicle of contamination. We also analysed the impact of the type of material used for the carrier (paper, baryta paper, cellulose acetate and nitrate or glass) or the light-sensitive layer (albumen, gelatine, collodion and other) on the level and diversity of microbial contamination. Carriers such as polyester and cellulose nitrate may have a negative impact on bacterial contamination, while paper and baryta paper may have a partially positive impact on both fungal and bacterial contamination.

【 授权许可】

Unknown