Transcriptome Changes and Potential Immunotoxicity Analysis in RAW264.7 Macrophages Caused by Bisphenol F

Frontiers in Pharmacology

Yungang Liu¹  Wei Zhang²  Li Li²  Huiling Chen²  Huanwen Tang²  Tianlan Li²  Xing Li²  Haoqi He²  Bohai Du²  Yanchao Zhang²  Ming Shi³ 

[1] Department of Toxicology, School of Public Health, Southern Medical University, Guangzhou, China;Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China;Dongguan Liaobu Hospital, Dongguan, China;

关键词: bisphenol F;    RAW264.7 macrophages;    immunotoxicity;    apoptosis;    transcription;   

DOI  :  10.3389/fphar.2022.846562

来源: DOAJ

【 摘 要 】

As a viable substitute for bisphenol A (BPA), BPF has been widely used in the plastic industry and daily consumer goods, resulting in its detection in humans at a comparable concentration. Evidence reveals that BPF and BPA may have similar toxic effects due to their similar structures. However, there is less information about BPF and its latent implications on the immune system, which is associated with many disorders. In this study, the in vitro toxicity of BPF on RAW264.7 macrophages was explored. The cells were treated with different concentrations of BPF (5, 10, 20, 50, 100, and 200 μM), the cell viability and apoptosis were detected, the gene expression profile was analyzed by whole-transcriptome sequencing, and the mRNA levels were detected by qRT-PCR. The results showed a high concentration of BPF could significantly reduce the survival rate of RAW264.7 macrophages. Although the medium concentration (20–50 μM) of BPF seemed to have no impact on the cell activity of macrophages, it caused the occurrence of apoptosis. The results of differential transcription showed that compared with the control group, 121 genes were upregulated and 82 genes were downregulated in the BPF group. The significantly changed gene functions were mainly concentrated in cell cycle, phagosome, lysosome, and antigen processing and presentation. These findings provide valuable information for correctly understanding the immunotoxicity risk of BPF and may help to improve the hazard identification of bisphenol compounds.

【 授权许可】

Unknown