Diverse biological effects of the essential oil from Iranian Trachyspermum ammi

Arabian Journal of Chemistry

Leonardo Sorci¹  Giuseppe Orsomando¹  Prosper C. Biapa Nya²  Onelia Bistoni³  Daniela Beghelli⁴  Dezemona Petrelli⁴  Luana Quassinti⁵  Giulio Lupidi⁵  Luca A. Vitali⁵  Massimo Bramucci⁵  Silvia Damiano⁵  Riccardo Petrelli⁵  Filippo Maggi⁵  Loredana Cappellacci⁵  Majid Majd Zadeh⁶  Fabrizio Papa⁷ 

[1] Department of Clinical Sciences, Section of Biochemistry, Polytechnic University of Marche, Ancona, Italy;Laboratory of Medicinal Plant Biochemistry, Food Science and Nutrition, Department of Biochemistry, Faculty of Sciences, University of Dschang, Dschang, Cameroon;Rheumatology Unit, Department of Medicine, University of Perugia, Perugia, Italy;School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy;School of Pharmacy, University of Camerino, Camerino, Italy;School of Plant Biology, Sapienza University, Rome, Italy;School of Science and Technology, University of Camerino, Camerino, Italy;

关键词: Trachyspermum ammi;    Ajwain oil;    Antimicrobial;    Antioxidant;    Cytotoxicity;    Immunostimulant;   

DOI  :  10.1016/j.arabjc.2015.06.002

来源: DOAJ

【 摘 要 】

Trachyspermum ammi (Apiaceae) is a plant with a good reputation in the traditional Persian and Ayurvedic medicine. The hydrodistilled essential oil from the fruits of T. ammi, known as ‘ajwain oil’, is used in countries such as Iraq, Iran, Afghanistan, Pakistan, and India in the preparation of curry, to flavour several foods, as preservative, and in perfumery. At therapeutic level, ajwain oil is employed in the treatment of gastrointestinal ailments, lack of appetite and bronchial problems. In the present work, the essential oil of T. ammi growing in Iran was analysed by GC–FID and GC–MS showing thymol (67.4%), p-cymene (17.9%) and γ-terpinene (11.3%) as the major constituents. Afterwards, we investigated the biological effects displayed by ajwain oil, namely the antimicrobial and antioxidant activity, the cytotoxicity on tumour cells, and the induction of lymphocyte proliferation. In addition, the inhibition on nicotinate mononucleotide adenylyltransferase (NadD), which is a promising new target for developing novel antibiotics, was evaluated. The antimicrobial effects of ajwain oil, measured by the agar disc diffusion method, were relevant, with inhibition zones higher than those of reference antibiotics, especially on Staphylococcus aureus and Candida albicans (34.7 and 54.3 mm, respectively). This effect was not due to the enzymatic inhibition on NadD. The ajwain oil exhibited a considerable dose-dependent inhibition on the ABTS radical cation, with an IC50 value of 22.4 μg/mL. MTT assay revealed that ajwain oil is particularly cytotoxic on colon carcinoma cells, with a IC50 value of 9.6 μg/mL. Finally, PBMC proliferation assay revealed some role for the ajwain oil within the network of interactions of the cells of the immune system.

【 授权许可】

Unknown