Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil

BMC Complementary and Alternative Medicine

Mohammad M Hudaib¹  Khaled A Tawaha¹  Sanaa K Bardaweel¹ 

[1] Department of Pharmaceutical Sciences, Faculty of Pharmacy, The University of Jordan, Queen Rania Street, Amman 11942, Jordan

关键词: Antiproliferative;    Antimicrobial;    Antioxidant;    Essential (volatile) oil;    Anthemis palestina;   

Others  :  1086989
DOI  :  10.1186/1472-6882-14-297

 received in 2014-01-15, accepted in 2014-08-04,  发布年份 2014

【 摘 要 】

Background

Anthemis palestina (Asteraceae) extends across the Mediterranean region, southwest Asia and eastern Africa. Although traditionally used for several applications, in vitro investigation of biological functions associated with Anthemis palestina essential oil had never been reported.

Methods

The air-dried flowers of Anthemis palestina were subjected to hydrodistillation to yield the oil. The antioxidant activity of the hydrodistilled oil was characterized using various in vitro model systems such as DPPH, ferric-reducing antioxidant power and hydroxyl radical scavenging activity. Antibacterial activity was tested against six bacterial species, representing both Gram positive and Gram negative bacteria. Antifungal activity was evaluated using three Candida species. The minimum inhibitory concentration (MIC) for each examined microorganism was determined using the microdilution method. The oil’s antiproliferative effects against eight human cancer cell lines were also studied and the lethal doses that resulted in 50% reduction of cell viability (LD₅₀) were determined.

Results

The results indicate that the essential oil of Anthemis palestina exhibited substantial antioxidant activities as demonstrated with DPPH, ferric reducing antioxidant power, and hydroxyl radical scavenging activity. In addition, a broad-spectrum antibacterial activity of the oil was revealed with better susceptibility of Gram positive bacteria towards the oil. The MIC values ranged between 6–75 μg/ml. Besides, the oil demonstrated a moderate inhibitory effect on the three Candida species examined; with MIC values ranging between 48–95 μg/ml. Potent cytotoxic activities, especially against HeLa cell line; with LD₅₀ of 32 μg/ml, BJAB cell line; with LD₅₀ of 57 μg/ml, and Caco-2 cell line; with LD₅₀ of 61 μg/ml, were observed.

Conclusion

The results obtained indicate high potential of Anthemis palestina essential oil as bioactive oil, for nutraceutical and medical applications, possessing antioxidant, antimicrobial and antiproliferative activities.

【 授权许可】

   
2014 Bardaweel et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

【 参考文献 】

• [1]Fernandes R: Genus Anthemis L. In Flora Europaea. Volume 4. Edited by Europaea F, Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb A. Cambridge, London: Cambridge University Press; 1976:145-159.
• [2]Bremer K, Humphries CJ: Generic Monograph of the Asteraceae-Anthemideae. B Nat Hist Mus 1993, 23:71-177.
• [3]Bremer K: Asteraceae, Cladistics and Classification. Portland, Oregon: Timber Press; 1994.
• [4]Javidnia K, Miri R, Kamalinejad M, Sarkarzadeh H, Jamalian A: Chemical composition of the essential oils of Anthemis altissima L. grown in Iran. Flavour Frag J 2004, 19:213-216.
• [5]Saroglou V, Dorizas N, Kypriotakis Z, Skaltsa HD: Analysis of the essential oil composition of eight Anthemi sspecies from Greece. J Chromatogr A 2006, 1104:313-322.
• [6]Rezaee MB, Jaimand K, Assareh MH: Chemical constituents of the leaf and flower oils from Anthemis altissima L. var. altissima from Iran. J Ess Oil Res 2006, 18:152-153.
• [7]Kivcak B, Mert T, Saglam H, Ozturk T, Kurkcuoglu M, Baser KHC: Chemical composition and antimicrobial activity of the essential oil of Anthemis wiedemanniana from Turkey. Chem Nat Comp 2007, 43:47-51.
• [8]Vaverkova S, Haban M, Eerna K: Qualitative properties of Anthemis tinctoria and Anthemis nobilis (Chamaemelum nobile) under different environmental conditions. J Cent Eur Agr 2001, 2:61-156.
• [9]Honda G, Yesilada E, Tabata M, Sezik E, Fujita T, Takeda Y, Tanaka T: Traditional medicine in Turkey. J Ethnopharmacol 1996, 53:75-87.
• [10]Konstantinopoulou M, Karioti A, Skaltsas S, Skaltsa H: Sesquiterpene lactones from Anthemis altissima and their anti-Helicobacter pylori activity. J Nat Prod 2003, 66:699-702.
• [11]Teixeira Da Silva JA: Mining the essential oils of the Anthemideae. African J Biotechnol 2004, 3:706-720.
• [12]Melegari M, Albasini A, Pecorari P, Vampa G, Rinaldi M, Rossi T, Bianchi A: Chemical characteristics and pharmacological properties of the essential oils of Anthemis nobilis. Fitoterapia 1988, 59:449-455.
• [13]Schrader A, Eckey H, Rohr M: Examination of the activity of irradiation-lowering substances on the human skin exemplified with various chamomile extracts. SOFW Journal 1997, 123:3-11.
• [14]Rudzki E, Jalblonska S: Kamillosan is a safe product of camomile for topical application: Results of patch testing consecutive patients with contact dermatitis. J Dermatol Treat 2000, 11:161-163.
• [15]Povilaityte V, Venskutonis PR, Jukneviciene G: Aroma and antioxidant properties of Roman chamomile (Anthemisnobilis L.). In Food Flavors and Chemistry: Advances of the New Millennium. Edited by Arthur MS. London, UK: Royal Society of Chemistry; 2001.
• [16]Teixeira DMC, Glyn MF, Adilson S, Vera LGR, Demarmelina C: Anti-Candida activity of Brazilian medicinal plants. J Ethnopharmacol 2005, 97:305-311.
• [17]Al-Eisawi DM: Field Guide to Wild Flowers of Jordan and Neighboring Countries. Foundation Al-Rai, Amman, Jordan: Jordan Press; 1998.
• [18]Tawaha KA, Alali FQ, Hudaib MM: Chemical Composition and General Cytotoxicity Evaluation of Essential Oil from the Flowers of Anthemis palestina Reut. Ex Boiss. Growing in Jordan. J Ess Oil Bear PlIn press
• [19]Sreejayan N, Rao MN: Free radical scavenging activity of curcuminoids. Drug Res 1996, 46:169-171.
• [20]Klein SM, Cohen G, Cederbaum AI: Production of formaldehyde during metabolism of Dimethyl sulphoxide by Hydroxyl Radical Generating System. Biochemistry 1981, 20:6006-6012.
• [21]Yen G, Chen H: Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 1995, 43:27-32.
• [22]Clinical Laboratory Standards Institute: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. In 7th edition. Edited by Wayne PA. 2006. Approved Standard M7-A7
• [23]Tubaro A, Florio C, Luxich E, Vertua R, Della Loggia R, Yasumoto T: Suitability of the MTT based cytotoxicity assay to detect okadaic acid contamination of mussels. Toxicon 1996, 34:965-974.
• [24]Antolovich M, Prenzler P, Patsalides E, McDonald S, Robards K: Methods for testing antioxidant activity. Analyst 2002, 127:183-198.
• [25]Dharmendra D, Prashant K, Jain SK: In-vitro Anti-oxidant activity of the ethyl acetate extract of gum guggul (Commiphora Mukul). Biological Forum-An Int J 2009, 1:32-35.
• [26]Erkan N, Ayranci G, Ayranci E: Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella Sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem 2008, 110:76-82.
• [27]Kelen M, Tepe B: Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresource Technol 2008, 99:4096-40104.
• [28]Maria Graça M: Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review. Molecules 2010, 15:9252-9287.
• [29]Meon M, Marchetti O, Calandra T: Bench-to bedside review: Candida infections in the intensive care unit. Crit Care 2008, 12:204-208.
• [30]Kamuhabwa A, Nshimo C, de Witte P: Cytotoxicity of some medicinal plant extracts used in Tanzanian traditional medicine. J Ethnopharmacol 2000, 70:143-149.
• [31]Moteki H, Hibasami H, Yamada Y, Katsuzaki H, Imai K, Komiya T: Specific induction ofapoptosis by 1,8-cineole in two human leukemia cell lines, but not a in human stomach cancer cell line. Oncology Rep 2002, 9:757-760.
• [32]Conforti F, Menichini F, Formisano C, Rigano D, Senatore F, Bruno M, Rosselli S, Celik S: Anthemis wiedemanniana essential oil prevents LPS-induced production of NO in RAW 264.7 macrophages and exerts antiproliferative and antibacterial activities in vitro. Nat Prod Res 2012, 26:1594-1601.
• [33]Lang G, Buchbauer G: A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review Flav Frag J 2012, 27:13-39.
• [34]Baratta MT, Dorman HJ, Deans SG, Figueiredo AC, Barroso JG, Ruperto G: Antimicrobial and antioxidant properties of some commercial essential oils. Flav Frag J 1998, 13:235-244.
• [35]Knoblach K, Pauli A, Iberi B, Wegand H, Weis N: Antibacterial and antifungal properties of essential oil components. J Ess Oil Res 1989, 1:119-128.
• [36]Bhardwaj P, Alok U, Khanna A: In vitro cytotoxicity of essential oils: A review. Int J Res Pharm Chem 2013, 3:675-681.
• [37]Ben Chobba I, Bekir A, Ben Mansour R, Drira N, Gharsallah N, Kadri A: In vitro evaluation of antimicrobial and cytotoxic activities of Rosmarinus officinalis L. (Lamiaceae) essential oil cultivated from south-west Tunisia. Appl Pharmaceut Sci 2012, 2:34-39.
• [38]Setzer WN, Schmidt JM, Noletto JA, Vogler B: Leaf oil compositions and bioactivities of abaco bush medicines. Pharmacologyonline 2006, 3:794-802.
• [39]Amorati R, Foti MC, Valgimigli L: Antioxidant Activity of Essential Oils. J Agric Food Chem 2013, 61:10835-10847.
• [40]Lee K-G, Shibamoto T: Determination of Antioxidant Potential of Volatile Extracts Isolated from Various Herbs and Spices. J Agric Food Chem 2002, 50:4947-4952.
• [41]Ruberto G, Baratta MT: Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem 2006, 69:167-174.
• [42]Shaaban H, El-Ghorab A, Shibamoto T: Bioactivity of essential oils and their volatile aroma components: Review. J Ess Oil Res 2012, 24:203-212.