In vitro antimicrobial, antioxidant and cytotoxic properties of Streptomyces lavendulae strain SCA5

BMC Microbiology

Savarimuthu Ignacimuthu⁴  Panthagani Praveen Kumar³  Chandrasekar Balachandran¹  Veeramuthu Duraipandiyan²  Naif Abdullah Al-Dhabi²  Pachaiyappan Saravana Kumar¹ 

[1] Division of Microbiology, Entomology Research Institute, Loyola College, Chennai 600 034, India;Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Department of Plant Biology and Biotechnology, Loyola College, Chennai 600 034, India;Visiting Professor Programme, College of Science, Deanship of Scientific Research, King Saud University, Riyadh, Saudi Arabia

关键词: Cytotoxicity;    Antioxidant;    Antimicrobial;    SCA5;   

Others  :  1137706
DOI  :  10.1186/s12866-014-0291-6

 received in 2014-09-19, accepted in 2014-11-10,  发布年份 2014

【 摘 要 】

Background

Actinomycetes are Gram-positive, often filamentous, bacteria known for their unsurpassed capacity for the production of secondary metabolites with diverse biological activities. The aim of the present study was to evaluate the antimicrobial, cytotoxic and antioxidant properties of Streptomyces lavendulae strain SCA5.

Results

The ethyl acetate extract of SCA5 broth (EA-SCA5) showed antimicrobial activity with MIC value of 31.25 μg/ml. EA-SCA5 showed good antioxidant potential by scavenging 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC₅₀ 507.61 ± 0.66 μg/ml), hydroxyl radical (IC₅₀ 617.84 ± 0.57 μg/ml), nitric oxide (IC₅₀ 730.92 ± 0.81 μg/ml) and superoxide anion radical (IC₅₀ 864.71 ± 1.15 μg/ml). The EA-SCA5 also showed strong suppressive effect on rat liver lipid peroxidation (IC₅₀ 838.83 ± 1.18 μg/ml). The total phenolic content of SCA5 was 577.12 mg of GAE equivalents/gram extract. EA-SCA5 exhibited cytotoxic activity on A549 adenocarcinoma lung cancer cell line. It showed 84.9% activity at 500 μg/ml with IC₅₀ value of 200 μg/ml. The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of one major bioactive compound actinomycin C2.

Conclusions

The results of this study indicate that the EA-SCA5 could be probed further for isolating some medically useful compounds.

【 授权许可】

   
2014 Saravana Kumar et al.; licensee BioMed Central Ltd.

【 图 表 】

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