Biophysical, Biochemical, and Molecular Docking Investigations of Anti-Glycating, Antioxidant, and Protein Structural Stability Potential of Garlic

Molecules

Subuhi Sherwani¹  Smita R. Verma²  Ritika Saha²  Mohd W. A. Khan³  Salma A. Al-Zahrani³  Ahmed A. Otaibi³  Abdulmohsen K. D. Alsukaibi³  Eida M. Alshammari³  Wahid A. Khan⁴  Nessar Ahmed⁵ 

[1] Department of Biology, College of Sciences, University of Ha’il, Ha’il 2440, Saudi Arabia;Department of Biotechnology, Delhi Technological University, Delhi 110042, India;Department of Chemistry, College of Sciences, University of Ha’il, Ha’il 2440, Saudi Arabia;Department of Clinical Biochemistry, College of Medicine, King Khalid University, Abha 61412, Saudi Arabia;Department of Life Sciences, Manchester Metropolitan University, Manchester M1 5GD, UK;

关键词: garlic;    antioxidant;    anti-glycation;    glycation;    AGEs;    HSA;   

DOI  :  10.3390/molecules27061868

来源: DOAJ

【 摘 要 】

Garlic has been reported to inhibit protein glycation, a process that underlies several disease processes, including chronic complications of diabetes mellitus. Biophysical, biochemical, and molecular docking investigations were conducted to assess anti-glycating, antioxidant, and protein structural protection activities of garlic. Results from spectral (UV and fluorescence) and circular dichroism (CD) analysis helped ascertain protein conformation and secondary structure protection against glycation to a significant extent. Further, garlic showed heat-induced protein denaturation inhibition activity (52.17%). It also inhibited glycation, advanced glycation end products (AGEs) formation as well as lent human serum albumin (HSA) protein structural stability, as revealed by reduction in browning intensity (65.23%), decrease in protein aggregation index (67.77%), and overall reduction in cross amyloid structure formation (33.26%) compared with positive controls (100%). The significant antioxidant nature of garlic was revealed by FRAP assay (58.23%) and DPPH assay (66.18%). Using molecular docking analysis, some of the important garlic metabolites were investigated for their interactions with the HSA molecule. Molecular docking analysis showed quercetin, a phenolic compound present in garlic, appears to be the most promising inhibitor of glucose interaction with the HSA molecule. Our findings show that garlic can prevent oxidative stress and glycation-induced biomolecular damage and that it can potentially be used in the treatment of several health conditions, including diabetes and other inflammatory diseases.

【 授权许可】

Unknown