Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms

STAR Protocols

Johnathan G. Crandall¹  Laura E. McKnight²  Scott D. Hansen²  Elizabeth T. Wiles²  Thomas B. Bailey²  Vi N. Truong²  Kona N. Orlandi²  Orion G.B. Banks²  Grace L. Waddell²  Eric U. Selker²  Jeffrey N. McKnight²  Drake A. Donovan² 

[1] Corresponding author;Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA;

关键词: Genomics;    Sequencing;    Model Organisms;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: MNase-seq (micrococcal nuclease sequencing) is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have developed a streamlined protocol for S. cerevisiae and other fungi which takes only three hours. Modified protocols were developed for wild fungi and mammalian cells. This method for rapidly producing sequencing-ready nucleosome footprints from several organisms makes MNase-seq faster and easier, with less chemical waste.

【 授权许可】

Unknown