In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

STAR Protocols

Stéphan Vagner¹  Hélène Malka-Mahieu²  Isabelle Girault³  Caroline Robert⁴  Shensi Shen⁵ 

[1] Corresponding author;INSERM U981, Gustave Roussy Cancer Campus, Villejuif, France;Singapore-Sichuan Frontier Medical Center, Sichuan University, Chengdu, China;INSERM U981, Gustave Roussy Cancer Campus, Villejuif, France;Institute of Thoracic Oncology, Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China;

关键词: Cell culture;    Cell-based Assays;    Microscopy;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The eukaryotic translation initiation complex eIF4F plays an important role in gene expression. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay-based procedure allowing the direct in situ visualization of the eIF4F complex, as well as its absolute quantification per cell using adapted image analysis software.For complete details on the use and execution of this protocol, please refer to Boussemart et al. (2014).

【 授权许可】

Unknown