An optimized protocol for the retroviral transduction of mouse CD4 T cells

STAR Protocols

Angel Porgador¹  Bishnu Khand²  Zoe V. Taylor³  Shir Zaccai⁴  Alon Monsonego⁵  Ekaterina Eremenko⁵ 

[1] Corresponding author;The Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel;The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel;The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel;The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel;

关键词: Cell culture;    Cell-based Assays;    Cell separation/fractionation;    Immunology;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death.For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019.

【 授权许可】

Unknown