Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions

STAR Protocols

Barbara Wegiel¹  Lubica Janovicova¹  Giacomo Canesin¹ 

[1] Department of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USA;

关键词: Cell Biology;    Cell-based Assays;    Cell separation/fractionation;    Cancer;    Protein Biochemistry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed protocol for detection of labile and total heme in prostate cancer cells and its measurement in subcellular compartments in vitro. The protocol can be adapted to be used for other cell types.For complete details on the use and execution of this protocol, please refer to Canesin et al. (2020).

【 授权许可】

Unknown