Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells

STAR Protocols

Rui-Ping Xiao¹  Li Zhi²  Lejiao Ren²  Peng Jiang³  Xinli Hu³ 

[1] Corresponding author;Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China;State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing 100871, China;

关键词: Cell Biology;    Cell culture;    Cell-based Assays;    Molecular Biology;    Gene Expression;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables sufficient and efficient gene expression for the downstream functional analysis.For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).

【 授权许可】

Unknown