Deconvolution of the hematopoietic stem cell microenvironment reveals a high degree of specialization and conservation

iScience

Patxi San Martin-Uriz¹  Ignacio Sancho-González¹  Delia Quilez Agreda¹  Miren Lasaga¹  Itziar Cenzano¹  David Gomez-Cabrero²  Jin Ye³  Felipe Prosper⁴  Borja Saez⁵  Jesper N. Tegner⁵  Nuria Planell⁵  Diego Alignani⁶  Bruno Paiva⁶  Isabel A. Calvo⁷  Luca Malcovati⁷  Gabriele Todisco⁷  Xabier Martinez-de-Morentin⁷  Amaia Vilas⁷  Marta Miñana Barrios⁷  Juan P. Romero⁷  Ana C. Viñado⁷ 

[1] Centro de Investigación Biomédica en Red de Cáncer, CIBERONC, Madrid, Spain;Unit of Precision Hematology Oncology, IRCCS S. Matteo Hospital Foundation, 27100 Pavia, Italy;Bioscience Program, Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology KAUST, Thuwal 23955, Saudi Arabia;;Department of Molecular Medicine, University of Pavia &Hospital Reina Sofía de Tudela, 31500 Navarra, Spain;Navarrabiomed, ComplejoHospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Pamplona, 31008 Navarra, Spain;Universidad de Navarra, CIMA, Hematology-Oncology Program, Instituto de Investigación Sanitaria de Navarra (IdiSNA), 31008 Navarra, Spain;

关键词: Biological sciences;    Stem cells research;    Omics;    Transcriptomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets. As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively. Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species. Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment.

【 授权许可】

Unknown