Microgravity-induced stress mechanisms in human stem cell-derived cardiomyocytes

iScience

Agapios Sachinidis¹  Ruth Hemmersbach²  Argyris Papantonis³  Eduardo Gade Gusmao³  Athanasia Mizi³  Toni Schneider⁴  Symeon Papadopoulos⁴  Felix Neumaier⁴  Aviseka Acharya⁴  Harshal Nemade⁴  Khadija Khan⁴  Jürgen Hescheler⁴  Krishna Rajendra Prasad⁴ 

[1] Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany;German Aerospace Center, Institute of Aerospace Medicine, Gravitational Biology, Linder Hoehe, 51147 Cologne, Germany;Institute of Pathology, University Medical Center Göttingen, 37077 Göttingen, Germany;University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany;

关键词: Biological sciences;    Molecular biology;    Cell biology;    Stem cells research;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Exposure to outer space microgravity poses a risk for the development of various pathologies including cardiovascular disease. To study this, we derived cardiomyocytes (CMs) from human-induced pluripotent stem cells and exposed them to simulated microgravity (SMG). We combined different “omics” and chromosome conformation capture technologies with live-cell imaging of various transgenic lines to discover that SMG impacts on the contractile velocity and function of CMs via the induction of senescence processes. This is linked to SMG-induced changes of reactive oxygen species (ROS) generation and energy metabolism by mitochondria. Taken together, we uncover a microgravity-controlled axis causing contractile dysfunctions to CMs. Our findings can contribute to the design of preventive and therapeutic strategies against senescence-associated disease.

【 授权许可】

Unknown